Co-immunoprecipitation as a Useful Tool for Detection of G Protein-Coupled Receptor Oligomers
Allosteric interactions between transmembrane G protein-coupled receptors (GPCRs) could lead to conformational changes and therefore oligomer function diversity increase. GPCR complexes are composed by homo- or heteroreceptors that can further assemble into receptor mosaic. These allosteric interactions could play a major role in brain regulation and plasticity. Alteration in GPCR neuromodulation may be involved in depression, schizophrenia, and addiction. Several studies reported that activation of the D4 dopaminergic receptor blocks many of the molecular, cellular, and behavioral effects produced by morphine. The existence of a MOR/D4R allosteric interaction through orthosteric agonist might lead to a secure therapeutic use of morphine. Here we describe the co-immunoprecipitation technique to study direct GPCR interaction in cell culture.
Key wordsG protein-coupled receptors Co-immunoprecipitation Addiction Morphine Dopamine 4 receptor μ-opioid receptor
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