Systems-Level Immune Monitoring by Mass Cytometry

  • Tadepally Lakshmikanth
  • Petter BrodinEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1913)


As therapies involving the modulation, stimulation, and deliberate excitation of the immune system are becoming routine, better methods for monitoring immune responses in human patients are needed. Mass cytometry allows for detailed profiling of all immune cell populations and their functional responses using a simple blood sample. When combined with appropriate computational analyses, the resolution for distinguishing desired responses from unproductive or even adverse reactions to immunotherapeutic interventions increases. Here we describe a core experimental and computational framework for global, systems-level immune monitoring by mass cytometry.

Key words

Mass cytometry Systems immunology CyTOF Human immunology Tumor immunology Immune monitoring 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Science for Life Laboratory, Division of Clinical Paediatrics, Department of Women’s and Children’s HealthKarolinska InstitutetStockholmSweden
  2. 2.Department of Newborn MedicineKarolinska University HospitalStockholmSweden

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