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Embryonic or Induced Pluripotent Stem Cell-Derived Hepatocellular Systems for HCV Culture

  • Xianfang WuEmail author
  • Viet Loan Dao ThiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1911)

Abstract

Human-induced pluripotent stem cell-derived hepatocyte-like cells (iHeps) constitute a powerful tool for modeling hepatotropic pathogen infections in cell culture. Meanwhile, CRISPR-Cas9 technology enables precise editing of stem cell genomes to generate patient-specific disease models and thus development of personalized experimental systems. Here we present a detailed stepwise protocol for the differentiation of stem cells to hepatocyte-like cells for HCV studies in cell culture. We also outline the use of an inducible iCRISPR platform for the rapid and efficient modification of host factors of interest to better understand their function during HCV infection.

Key words

Human embryonic/induced pluripotent stem cells Hepatocyte-like cells iCRISPR Precision medicine 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Virology and Infectious Diseases, Center for the Study of Hepatitis CThe Rockefeller UniversityNew YorkUSA
  2. 2.Center for Integrative Infectious Disease ResearchUniversity Hospital HeidelbergHeidelbergGermany
  3. 3.Laboratory of Virology and Infectious DiseasesCenter for the Study of Hepatitis C, The Rockefeller UniversityNew YorkUSA

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