Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy
Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.
Key wordsMicroscopy Super-resolution Imaging Virus Hepatitis
We would like to thank Ricardo Henriques and Lucas Walker for advice and technical support. JG is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (107653/Z/15/Z). PMP is supported by a Biotechnology and Biological Sciences Research Council grant (BB/M022374/1). CJ is a Commonwealth Scholar, funded by the UK government. The imaging systems used in this work were funded by the Medical Research Council (MR/K015826/1).
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