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Cell-Free DNA: An Overview of Sample Types and Isolation Procedures

  • Filippo Martignano
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1909)

Abstract

The study of cell-free DNA (cfDNA) is often challenging due to genomic DNA contamination, low concentration, and high fragmentation. Therefore, it is important to optimize pre-analytical and analytical procedures in order to maximize the performance of cfDNA-based analyses.

In this chapter, we report the most common methods for the correct collection, centrifugation, storage, and DNA isolation from cell-free biological sources such as plasma, urines, cerebrospinal fluid, and pleural effusion fluid.

Key words

Cell-free DNA Plasma Serum Urines Cerebrospinal fluid Pleural effusion fluid Collection Storage Centrifugation Nucleic acid extraction 

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Filippo Martignano
    • 1
  1. 1.Department of Medical BiotechnologiesUniversity of SienaSienaItaly

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