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Urinary Cell-Free DNA: Isolation, Quantification, and Quality Assessment

Part of the Methods in Molecular Biology book series (MIMB,volume 1909)

Abstract

Urine cell-free DNA is an important source of diagnostic markers for different diseases (e.g., cancer and prenatal diagnosis). It is important to achieve a simple and fast protocol to maximize the recovery of DNA from urine supernatant and to assess its quality. Here we describe a simple approach from urine collection to DNA quality assessment for downstream analyses.

Key words

  • Urine
  • Cell-free DNA
  • Isolation
  • Quantification
  • Quality
  • Protocol

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  • DOI: 10.1007/978-1-4939-8973-7_16
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Casadio, V., Salvi, S. (2019). Urinary Cell-Free DNA: Isolation, Quantification, and Quality Assessment. In: Casadio, V., Salvi, S. (eds) Cell-free DNA as Diagnostic Markers. Methods in Molecular Biology, vol 1909. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8973-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8973-7_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8972-0

  • Online ISBN: 978-1-4939-8973-7

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