Skip to main content
Book cover

Cell-free DNA as Diagnostic Markers pp 211–221Cite as

Urinary Cell-Free DNA: Isolation, Quantification, and Quality Assessment

Part of the Methods in Molecular Biology book series (MIMB,volume 1909)

Abstract

Urine cell-free DNA is an important source of diagnostic markers for different diseases (e.g., cancer and prenatal diagnosis). It is important to achieve a simple and fast protocol to maximize the recovery of DNA from urine supernatant and to assess its quality. Here we describe a simple approach from urine collection to DNA quality assessment for downstream analyses.

Key words

  • Urine
  • Cell-free DNA
  • Isolation
  • Quantification
  • Quality
  • Protocol

This is a preview of subscription content, access via your institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-4939-8973-7_16
  • Chapter length: 11 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   139.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-8973-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book
USD   179.99
Price excludes VAT (USA)
Learn about institutional subscriptions
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Ponti G, Maccaferri M, Manfredini M et al (2018) The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients. Clin Chim Acta 479:14–19

    CAS  CrossRef  PubMed  Google Scholar 

  2. Bartels S, Persing S, Hasemeier B et al (2017) Molecular analysis of circulating cell-free DNA from lung cancer patients in routine laboratory practice: a cross-platform comparison of three different molecular methods for mutation detection. J Mol Diagn 19(5):722–732

    CAS  CrossRef  PubMed  Google Scholar 

  3. Lu T, Li J (2017) Clinical applications of urinary cell-free DNA in cancer: current insights and promising future. Am J Cancer Res 7(11):2318–2332

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Salvi S, Martignano F, Molinari C et al (2016) The potential use of urine cell free DNA as a marker for cancer. Expert Rev Mol Diagn 16(12):1283–1290

    CAS  CrossRef  PubMed  Google Scholar 

  5. Salvi S, Gurioli G, Martignano F et al (2015) Urine cell-free DNA integrity analysis for early detection of prostate cancer patients. Dis Markers 2015:574120

    CrossRef  PubMed  PubMed Central  Google Scholar 

  6. Casadio V, Calistri D, Tebaldi M et al (2013) Urine cell-free DNA integrity as a marker for early bladder cancer diagnosis: preliminary data. Urol Oncol 31(8):1744–1750

    CAS  CrossRef  PubMed  Google Scholar 

  7. Sands J, Li Q, Hornberger J (2017) Urine circulating-tumor DNA (ctDNA) detection of acquired EGFR T790M mutation in non-small-cell lung cancer: an outcomes and total cost-of-care analysis. Lung Cancer 110:19–25

    CrossRef  PubMed  Google Scholar 

  8. Botezatu I, Serdyuk O, Potapova G et al (2000) Genetic analysis of DNA excreted in urine: a new approach for detecting specific genomic DNA sequences from cells dying in an organism. Clin Chem 46(8 Pt 1):1078–1084

    CAS  PubMed  Google Scholar 

  9. Majer S, Bauer M, Magnet E, Strele A et al (2007) Maternal urine for prenatal diagnosis—an analysis of cell-free fetal DNA in maternal urine and plasma in the third trimester. Prenat Diagn 27(13):1219–1223

    CAS  CrossRef  PubMed  Google Scholar 

  10. Tsui NB, Jiang P, Chow KC et al (2012) High resolution size analysis of fetal DNA in the urine of pregnant women by paired-end massively parallel sequencing. PLoS One 7(10):e48319

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  11. Lin SY, Linehan JA, Wilson TG et al (2017) Emerging utility of urinary cell-free nucleic acid biomarkers for prostate, bladder, and renal cancers. Eur Urol Focus 3(2–3):265–272

    CrossRef  PubMed  Google Scholar 

  12. Casadio V, Salvi S, Martignano F et al (2017) Cell-free DNA integrity analysis in urine samples. J Vis Exp (119)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy

    Valentina Casadio & Samanta Salvi

Authors
  1. Valentina Casadio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Samanta Salvi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Biosciences Laboratory, IRCCS I.R.S.T, Meldola, Italy

    Valentina Casadio

  2. Biosciences Laboratory, IRCCS I.R.S.T, Meldola, Italy

    Samanta Salvi

Rights and permissions

Reprints and Permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Verify currency and authenticity via CrossMark

Cite this protocol

Casadio, V., Salvi, S. (2019). Urinary Cell-Free DNA: Isolation, Quantification, and Quality Assessment. In: Casadio, V., Salvi, S. (eds) Cell-free DNA as Diagnostic Markers. Methods in Molecular Biology, vol 1909. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8973-7_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8973-7_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8972-0

  • Online ISBN: 978-1-4939-8973-7

  • eBook Packages: Springer Protocols