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Urinary Cell-Free DNA: Isolation, Quantification, and Quality Assessment

  • Valentina Casadio
  • Samanta Salvi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1909)

Abstract

Urine cell-free DNA is an important source of diagnostic markers for different diseases (e.g., cancer and prenatal diagnosis). It is important to achieve a simple and fast protocol to maximize the recovery of DNA from urine supernatant and to assess its quality. Here we describe a simple approach from urine collection to DNA quality assessment for downstream analyses.

Key words

Urine Cell-free DNA Isolation Quantification Quality Protocol 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Valentina Casadio
    • 1
  • Samanta Salvi
    • 1
  1. 1.Biosciences LaboratoryIstituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCSMeldolaItaly

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