Cell-Free DNA: Applications in Different Diseases

  • Rossella Ranucci
Part of the Methods in Molecular Biology book series (MIMB, volume 1909)


Since its discovery in human blood plasma about 70 years ago, circulating cell-free DNA (cfDNA) has become an attractive subject of research as noninvasive disease biomarker. The interest in clinical applications has gained an exponential increase, making it a popular and potential target in a wide range of research areas.

cfDNA can be found in different body fluids, both in healthy and not healthy subjects. The recent and rapid development of new molecular techniques is promoting the study and the identification of cfDNA, holding the key to minimally invasive diagnostics, improving disease monitoring, clinical decision, and patients’ outcome.

cfDNA has already given a huge impact on prenatal medicine, and it could become, in the next future, the standard of care also in other fields, from oncology to transplant medicine and cardiovascular diseases.

Key words

Cell-free DNA Liquid biopsy Cell-free fetal DNA Circulating tumor DNA Clinical application 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rossella Ranucci
    • 1
  1. 1.Biosciences LaboratoryIstituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCSMeldolaItaly

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