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Micellar Electrokinetic Chromatography

  • Braden C. Giordano
  • Ronald Siefert
  • Greg E. Collins
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1906)

Abstract

Micellar electrokinetic chromatography (MEKC) is a mode of capillary electrophoresis that allows for the separation of neutral molecules in an electric field. Typically, neutral molecules move with electroosmotic flow (EOF) or bulk flow during electrophoretic separations resulting in no temporal resolution between mixtures of neutral analytes. Inclusion of surfactant micelles in the separation buffer allows for the separation of neutral analytes from one another through association with the micelle. Here we outline the implementation of MEKC for the separation of neutral molecules using a mixture of nitroaromatic explosives and their degradation products serving as a test analyte mixture.

Key words

Electrophoresis Preconcentration Explosives 

References

  1. 1.
    Terabe S, Otsuka K, Ichikawa K, Tsuchiya A, Ando T (1984) Electrokinetic separations with micellar solutions and open-tubular capillaries. Anal Chem 56:111–113CrossRefGoogle Scholar
  2. 2.
    Quirino JP, Kim JB, Terabe S (2002) Sweeping: concentration mechanism and applications to high-sensitivity analysis in capillary electrophoresis. J Chromatogr A 965:357–373CrossRefGoogle Scholar
  3. 3.
    Quirino JP, Terabe S (1998) Exceeding 5000-fold concentration of dilute analytes in micellar electrokinetic chromatography. Science 282:465–468CrossRefGoogle Scholar
  4. 4.
    Quirino JP, Terabe S (1999) Sweeping of analyte zones in electrokinetic chromatography. Anal Chem 71:1638–1644CrossRefGoogle Scholar
  5. 5.
    Palmer J, Munro NJ, Landers JP (1999) A universal concept for stacking neutral analytes in micellar capillary electrophoresis. Anal Chem 71:1679–1687CrossRefGoogle Scholar
  6. 6.
    Palmer J, Burgi DS, Landers JP (2002) Electrokinetic stacking injection of neutral analytes under continuous conductivity conditions. Anal Chem 74:632–638CrossRefGoogle Scholar
  7. 7.
    Liu ZY, Sam P, Sirimanne SR, Mcclure PC, Grainger J, Patterson DG (1994) Field-amplified sample stacking in micellar electrokinetic chromatography for on-column sample concentration of neutral molecules. J Chromatogr A 673:125–132CrossRefGoogle Scholar
  8. 8.
    Giordano BC, Newman CID, Federowicz PM, Collins GE, Burgi DS (2007) Micelle stacking in micellar electrokinetic chromatography. Anal Chem 79:6287–6294CrossRefGoogle Scholar
  9. 9.
    Quirino JP, Terabe S (1998) On line concentration of neutral analytes for micellar electrokinetic chromatography. 5. Field enhanced sample injection with reverse migrating micelles. Anal Chem 70:1893–1901CrossRefGoogle Scholar
  10. 10.
    Quirino JP, Terabe S (1998) On-line concentration of neutral analytes for micellar electrokinetic chromatography—IV. Field-enhanced sample injection. J Chromatogr A 798:251–257CrossRefGoogle Scholar
  11. 11.
    Quirino JP, Otsuka K, Terabe S (1998) On-line concentration of neutral analytes for micellar electrokinetic chromatography—VI. Stacking using reverse migrating micelles and a water plug. J Chromatogr B 714:29–38CrossRefGoogle Scholar
  12. 12.
    Quirino JP, Terabe S (1998) On-line concentration of neutral analytes for micellar electrokinetic chromatography. 3. Stacking with reverse migrating micelles. Anal Chem 70:149–157CrossRefGoogle Scholar
  13. 13.
    Kim JB, Otsuka K, Terabe S (2001) On-line sample concentration in micellar electrokinetic chromatography with cationic micelles in a coated capillary. J Chromatogr A 912:343–352CrossRefGoogle Scholar
  14. 14.
    Monton MRN, Otsuka K, Terabe S (2003) On-line sample preconcentration in micellar electrokinetic chromatography by sweeping with anionic-zwitterionic mixed micelles. J Chromatogr A 985:435–445CrossRefGoogle Scholar
  15. 15.
    Monton MRN, Quirino JP, Otsuka K, Terabe S (2001) Separation and on-line preconcentration by sweeping of charged analytes in electrokinetic chromatography with nonionic micelles. J Chromatogr A 939:99–108CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Braden C. Giordano
    • 1
  • Ronald Siefert
    • 2
  • Greg E. Collins
    • 1
  1. 1.Chemistry DivisionU.S. Naval Research LaboratoryWashingtonUSA
  2. 2.Chemistry Department United States Naval AcademyAnnapolisUSA

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