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Sample Preconcentration Protocols in Microfluidic Electrophoresis

  • Fumihiko Kitagawa
  • Koji Otsuka
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1906)

Abstract

Electrophoretic on-line sample preconcentration techniques in microfluidic channels improve the sensitivity prior to the separation. Among various techniques, the most important field-amplified sample stacking and sweeping on cross-channel microchips are demonstrated. As a novel microfluidic preconcentration approach, a large-volume sample stacking with electroosmotic flow pump (LVSEP) on straight-channel chips is also presented, which can omit a complicated voltage program for sample injection processes. In this chapter, we describe how to prepare and how to run these on-line sample preconcentration methods in microchip electrophoresis.

Key words

Microchip electrophoresis On-line sample preconcentration Field-amplified sample stacking Sweeping LVSEP 

Notes

Acknowledgments

We would like to thank Dr. Kenji Sueyoshi (Osaka Prefecture University, Japan) and Dr. Takayuki Kawai (RIKEN, Japan) for collaborating the sweeping and LVSEP experiments, respectively. This work was supported in part by the Grant-in-Aid for Scientific Research (C) (No. 24550090 and 15K05527) from the Japan Society for the Promotion of Science (JSPS). This research was also supported by SENTAN, JST.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Frontier Materials Chemistry, Graduate School of Science and TechnologyHirosaki UniversityHirosakiJapan
  2. 2.Department of Material Chemistry, Graduate School of EngineeringKyoto UniversityKyotoJapan

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