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Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration

  • Sungjin Ko
  • Donghun Shin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)

Abstract

Following massive hepatocyte ablation in zebrafish, biliary epithelial cells can extensively give rise to hepatocytes through liver progenitor cells (LPCs). The zebrafish liver injury model is an important system to elucidate the molecular mechanisms underlying LPC-driven liver regeneration. Here, we describe a chemical screening method using the zebrafish model for identifying small molecules that can modulate LPC-driven liver regeneration.

Key words

Nitroreductase Biliary epithelial cells Hepatocyte ablation Chemical screen Liver progenitor cells 

Notes

Acknowledgments

We thank Jackie Russell, Mehwish Khaliq, and Michael Tsang for editing the manuscript. This study was supported by an NIH grant (DK101426) to D.S.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Developmental BiologyMcGowan Institute for Regenerative Medicine, Pittsburgh Liver Research Center, University of PittsburghPittsburghUSA

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