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Analysis for Remodeling of Hepatic Tissue Structures in 3D During Regeneration

  • Kota Kaneko
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)

Abstract

It has been demonstrated that the liver remarkably alter its tissue structures during regeneration, and various types of liver stem or progenitor cells locating in specific areas in the liver tissue contribute to regeneration. Therefore, it is important to analyze the dynamic rearrangement of the liver tissue structures in 3D for better understanding the process and mechanism of liver regeneration. Here we describe a macroscopic analysis method to visualize the whole 3D structure of the vasculatures and the biliary tree, which are dynamically remodeled during regeneration, and a microscopic analysis method to visualize detailed structures at the cellular level, which can compensate what cannot be detected in the macroscopic analysis.

Key words

Liver regeneration Liver pathology 3D tissue structure 3D imaging Ductular reaction Biliary tree 

Notes

Acknowledgments

The experiments to establish these methods were supported in part by Japan Society for the Promotion of Science KAKENHI grants 22118006 (to Dr. Atsushi Miyajima), 24112507 (to Dr. Tohru Itoh), 24590342 (to T.I.), and 26253023 (to A.M.) as well as by CREST from the Japan Science and Technology Agency (to A.M.). K. K. was a research fellow at the Japan Society for the Promotion of Science.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pathology, School of MedicineUniversity of CaliforniaSan DiegoUSA

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