Analysis for Remodeling of Hepatic Tissue Structures in 3D During Regeneration
It has been demonstrated that the liver remarkably alter its tissue structures during regeneration, and various types of liver stem or progenitor cells locating in specific areas in the liver tissue contribute to regeneration. Therefore, it is important to analyze the dynamic rearrangement of the liver tissue structures in 3D for better understanding the process and mechanism of liver regeneration. Here we describe a macroscopic analysis method to visualize the whole 3D structure of the vasculatures and the biliary tree, which are dynamically remodeled during regeneration, and a microscopic analysis method to visualize detailed structures at the cellular level, which can compensate what cannot be detected in the macroscopic analysis.
Key wordsLiver regeneration Liver pathology 3D tissue structure 3D imaging Ductular reaction Biliary tree
The experiments to establish these methods were supported in part by Japan Society for the Promotion of Science KAKENHI grants 22118006 (to Dr. Atsushi Miyajima), 24112507 (to Dr. Tohru Itoh), 24590342 (to T.I.), and 26253023 (to A.M.) as well as by CREST from the Japan Science and Technology Agency (to A.M.). K. K. was a research fellow at the Japan Society for the Promotion of Science.
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