Generation of Hepatic Organoids with Biliary Structures
Incorporation of bile drainage system into engineered liver tissue is an important issue to advance liver regenerative medicine. Our group reported that three-dimensional (3D) coculture of fetal liver cells (FLCs) and adult rat biliary epithelial cells (BECs) allows reconstruction of hepatic spheroids that possess bile ductular structures. In this chapter, we describe the detailed protocol to isolate FLCs and BECs and to generate the spheroids with bile drainage system using these two types of primary cells.
Key wordsBiliary epithelial cell Fetal liver cell Bile ducts Hetero-spheroid 3D culture Tissue engineering
This work was supported in part by Grant-in-Aid for Young Scientists B (16K16643) and the Research Program on Hepatitis (16fk0310505h0005) from the Japan Agency for Medical Research and Development (AMED).
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