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Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture

  • Fumiya Tao
  • Hirotaka Mihara
  • Nobuhiko Kojima
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)

Abstract

The hepatic functions of the hepatocytes in multicellular spheroid (MCS) are lower than those in the liver. One of the causes is that conventional hepatic MCSs do not reproduce liver-specific microstructures such as hepatic cord. It is necessary to design the inner structure of hepatic MCSs mimicking a structural feature of hepatic cord to represent further hepatic functions. Here we introduce a unique method to engineer the microarchitectures in the MCSs by formation of void spaces or filling of extracellular matrices (ECMs).

Key words

Multicellular spheroid Hepatocyte Methylcellulose Microstructure Alginate hydrogel bead Extracellular matrix 

Notes

Acknowledgment

This work was supported by JSPS KAKENHI under Grant Number 24710142 and AMED under Grant Number JP17be034302.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Life and Environmental System Science, Graduate School of NanobioscienceYokohama City UniversityYokohamaJapan

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