Induction of Functional Hepatocytes from Human iPSCs

  • Taketomo KidoEmail author
  • Yuta KouiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)


Human-induced pluripotent stem cells (iPSCs) could be a useful source for production of hepatocytes. Here, we develop protocols to generate iPSC-derived liver progenitor cells, liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs). We also establish long-term two-dimensional co-culture system to induce functional hepatocytes from iPSC-derived liver cells.

Key words

Human-induced pluripotent stem cells Liver Liver progenitor cells Hepatocytes Liver sinusoidal endothelial cells Hepatic stellate cells 



This study was supported by CREST program of Japan Science and Technology Agency, Grants-in-Aid for Scientific Research of Japan Society for the Promotion of Science, and Japan Agency for Medical Research and Development (AMED).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan

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