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Induction of Functional Hepatocytes from Human iPSCs

  • Taketomo Kido
  • Yuta Koui
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)

Abstract

Human-induced pluripotent stem cells (iPSCs) could be a useful source for production of hepatocytes. Here, we develop protocols to generate iPSC-derived liver progenitor cells, liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs). We also establish long-term two-dimensional co-culture system to induce functional hepatocytes from iPSC-derived liver cells.

Key words

Human-induced pluripotent stem cells Liver Liver progenitor cells Hepatocytes Liver sinusoidal endothelial cells Hepatic stellate cells 

Notes

Acknowledgment

This study was supported by CREST program of Japan Science and Technology Agency, Grants-in-Aid for Scientific Research of Japan Society for the Promotion of Science, and Japan Agency for Medical Research and Development (AMED).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan

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