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Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo

  • Guangqi Song
  • Qinggong Yuan
  • Zhen Dai
  • Hsin-Chieh Tsay
  • Xizhong Shen
  • Michael Ott
  • Amar Deep Sharma
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)

Abstract

In vivo conversion of fibroblasts into hepatocyte-like cells provides one potential approach for the treatment of liver fibrosis. In our previous study, we showed in vivo conversion of myofibroblasts into induced hepatocytes (iHeps) by forced expression of four transcription factors in genetic fate-tracing mouse model of chronic liver disease. These in vivo-generated iHeps showed similar expression profile with endogenous hepatocytes (eHeps) and also exhibited similar functional characteristics, such as albumin secretion, urea synthesis, cytochrome activity, and drug responsiveness. Furthermore, the targeted expression of our reprogramming factors in myofibroblasts attenuated liver fibrosis. Our study suggests that in vivo reprogramming may open new perspectives for the treatment of diseases such as liver fibrosis.

Key words

In vivo reprogramming Induced hepatocytes (iHeps) 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Guangqi Song
    • 1
  • Qinggong Yuan
    • 2
  • Zhen Dai
    • 2
  • Hsin-Chieh Tsay
    • 2
  • Xizhong Shen
    • 1
  • Michael Ott
    • 2
  • Amar Deep Sharma
    • 2
  1. 1.Department of Gastroenterology, Zhongshan HospitalFudan UniversityShanghaiChina
  2. 2.Department of Gastroenterology, Hepatology and EndocrinologyHannover Medical SchoolHannoverGermany

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