Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells

  • Atsunori TsuchiyaEmail author
  • Shuji Terai
Part of the Methods in Molecular Biology book series (MIMB, volume 1905)


Mouse fetal liver includes abundant hepatic stem/progenitor cells (HSPCs). Easy expansion with passage of HSPCs is necessary to obtain steady data. However, it is often difficult to enrich only HSPCs, and HSPCs can die when usual trypsin is used for replating. Here, we introduce serum-free long-term culture with passage of HSPCs using fetal mouse liver without a cell sorter.

Key words

Hepatic progenitor cell Spheroid Serum-free culture Colony Mouse 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Gastroenterology and HepatologyGraduate School of Medical and Dental Science, Niigata UniversityNiigataJapan

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