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Pollen Tube Pathway-Mediated Cotton Transformation

  • Min Wang
  • Runrun Sun
  • Baohong ZhangEmail author
  • Qinglian Wang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1902)

Abstract

Although many gene transfer methods have been employed for successfully obtaining transgenic cotton, the major constraint in cotton improvement is the limitation of genotype because the majority of transgenic methods require plant regeneration from a single transformed cell which is limited by cotton tissue culture. Comparing with other plant species, it is difficult to induce plant regeneration from cotton; currently, only a limited number of cotton cultivars can be cultured for obtaining regenerated plants. Thus, developing a simple and genotype-independent genetic transformation method is particularly interested for cotton. In this chapter, we present a simple, cost-efficient, and genotype-independent cotton transformation method – pollen tube pathway-mediated transformation. This method uses pollen tube pathway to deliver transgene into cotton embryo sacs and then insert foreign genes into cotton genome. There are three major steps for pollen tube pathway-mediated genetic transformation, which include injection of foreign genes into pollen tube, integration of foreign genes into plant genome, and selection of transgenic plants.

Key words

Cotton Transformation Pollen tube pathway Genotype-independent 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Min Wang
    • 1
  • Runrun Sun
    • 2
  • Baohong Zhang
    • 3
    Email author
  • Qinglian Wang
    • 2
  1. 1.Beijing Key Laboratory of Plant Resources Research and DevelopmentBeijing Technology and Business UniversityBeijingChina
  2. 2.Henan Collaborative Innovation Center of Modern Biological BreedingHenan Institute of Sciences and TechnologyXinxiangChina
  3. 3.Department of BiologyEast Carolina UniversityGreenvilleUSA

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