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A Grafting Technique for Efficiently Transplanting Transgenic Regenerated Plants of Cotton

  • Min Wang
  • Qinglian Wang
  • Baohong ZhangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1902)

Abstract

Recovery of transgenic cotton plants from tissue culture condition to greenhouse condition is a critical step for improving cotton through genetic engineering. Traditional methods always cause low survival rate of transplanted plants. In 1998, we developed an efficient grafting technique for recovery of transgenic cotton plants, which significantly increased the survival rate of the transplanting regeneration plants. In this chapter, we present a detailed protocol for grafting transgenic cotton plants obtaining somatic embryogenesis and transgenics.

Key words

Transgenic cotton Plant regeneration Graft Grafting Gossypium hirsutum L. 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Plant Resources Research and DevelopmentBeijing Technology and Business UniversityBeijingChina
  2. 2.Henan Collaborative Innovation Center of Modern Biological BreedingHenan Institute of Sciences and TechnologyXinxiangChina
  3. 3.Department of BiologyEast Carolina UniversityGreenvilleUSA

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