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Screening of Transgenic Cotton Based on a Porous Silicon Biosensor

  • Liangliang Chen
  • Peng Li
  • Xiaoyi Lv
  • Ji MaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1902)

Abstract

Label-free porous silicon (PSi)-based biosensor enables the detection and identification of the protein in transgenic cotton. Changes in optical response signal in the presence of the target protein can be detected by Fourier transform infrared (FTIR) spectromicroscopy when binding of the target protein with its antibody is selectively captured on the PSi biosensor. Here we describe the transgenic protein (antifreeze protein, AFP), and compare with non-transgenic plants. Significant red shifts are observed for transgenic antifreeze protein cotton lines.

Key words

Transgenic cotton Antibody Porous silicon Protein biosensor 

Notes

Acknowledgment

This work was supported by NSFC grant (No. 31060292) and a key grant from the Ministry of Agriculture of China (No. 2009ZX08005-022B).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Life Science and TechnologyXinjiang UniversityÜrümqiChina
  2. 2.College of Information Science and EngineeringXinjiang UniversityÜrümqiChina

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