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Development of an Enzyme-Linked Immunosorbent Assay for the Detection of GM Proteins in Transgenic Crops/Produce

  • Suchitra KamleEmail author
  • Dawei Li
  • Abhishek Ojha
  • Arvind Kumar
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1902)

Abstract

Enzyme-linked immunosorbent assays are always being used extensively for the identification of genetically modified protein in transgenic crops/produce. With the advancement in the detection strategies, competitive and sandwich immunoassays are more convenient and sensitive for the GM protein detection and its quantification in transgenic crops. This chapter is focusing on the competitive ELISA including sandwich ELISA for the detection of the GM proteins.

Key words

Antibody Genetically modified Immunoassay Transgenics 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Suchitra Kamle
    • 1
    Email author
  • Dawei Li
    • 2
  • Abhishek Ojha
    • 3
  • Arvind Kumar
    • 4
  1. 1.Microbiology, Molecular & ImmunologyBrown UniversityProvidenceUSA
  2. 2.School of PharmacyShanghai Jiao Tong UniversityShanghaiChina
  3. 3.Structural and Computational Biology LabInternational Centre for Genetic Engineering and BiotechnologyNew DelhiIndia
  4. 4.Faculty of Science, Molecular Biology and Immunology Lab, School of BiotechnologyBanaras Hindu UniversityVaranasiIndia

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