Autoantibodies pp 177-182 | Cite as

Analysis of C3 Nephritic Factors by ELISA

  • Lillemor Skattum
Part of the Methods in Molecular Biology book series (MIMB, volume 1901)


Autoantibodies to the C3 convertase of the alternative pathway of complement, called C3 nephritic factors (C3NeF), cause persistently low C3 in the circulation and production of C3 degradation fragments due to prolonged stabilization of the C3 convertase. C3NeF are associated with glomerulopathy, acquired partial lipodystrophy, and less frequently with increased susceptibility to meningococcal infection. Analysis of C3NeF is an important part of the diagnostic workup of C3 glomerulopathy, but their identification is difficult presumably due to considerable heterogeneity. Therefore it is recommended to use a combination of different analysis methods for the detection of C3NeF. Here we present an ELISA method for detection of C3NeF.

Key words

Autoantibodies to complement C3 nephritic factors ELISA C3 glomerulopathy Alternative pathway convertase 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Laboratory Medicine, Section of Microbiology, Immunology and GlycobiologyLund UniversityLundSweden
  2. 2.Clinical Immunology and Transfusion MedicineRegion SkåneSweden

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