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Culturing of Rodent Brain Microvascular Endothelial Cells for In Vitro Modeling of the Blood-Brain Barrier

  • Malgorzata Burek
  • Carola Y. Förster
Protocol
Part of the Neuromethods book series (NM, volume 142)

Abstract

The blood-brain barrier (BBB) is important in the maintenance of the microenvironment of the brain and proper neuronal function. Apart from the protective function, BBB regulates entry of nutrients into the central nervous system by selective transport and metabolism of blood- and brain-borne substances. Successful modeling of BBB in vitro is established since 1970s and has been used to study mechanisms of transport, cellular interaction, and gene regulation. Rodent in vitro BBB models are widely used and have been proven to retain sufficiently the in vivo properties during culturing. In this chapter we describe methodological aspects of culturing the microvascular endothelial cells. Immortalized endothelial cell lines as well as primary brain microvascular endothelial cells in monoculture, co-culture, and triple-culture are discussed.

Key words

Blood-brain barrier Brain microvascular endothelial cells In vitro model TEER 

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Anaesthesia and Critical CareUniversity of WürzburgWürzburgGermany

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