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Biomarkers for Microvascular Proteins Detection: Blood–Brain Barrier Injury and Damage Measurement

  • Pavani Sayana
  • Jean Pierre Oses
  • Tatiana Barichello
  • Vijayasree V. GiridharanEmail author
Protocol
Part of the Neuromethods book series (NM, volume 142)

Abstract

Blood–brain barrier (BBB) is a highly selective semipermeable membrane with tight junctions formed from closely wedged epithelial cells and is the principal regulator of exchange of materials between the blood and the brain, simultaneously protecting it from the systemic insults. Any impairment in the functional coordinated effort or formation of the components of the BBB neurovascular bundle may lead to neurological disturbances from entry of extraneous elements. The BBB disruption causes an imbalance in tight junction proteins, adherens junction proteins, matrix metalloproteinases, cell adhesion molecules, and a myriad of neuroinflammatory proteins. Therefore, the modalities, which can enable the accurate detection of BBB breach in advance via protein biomarker assay to underpin targeted medicines, are of growing priority. In this chapter, we will summarize the BBB pathology measurement methods used in the detection of microvascular protein biomarkers such as Western blot, immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), and real-time polymerase chain reaction (RT-PCR). In addition, we also emphasize on the BBB genomics and the method used to detect functionally related BBB genes in preclinical model.

Key words

Blood–brain barrier Brain microvasculature Tight junction proteins Western blot Immunohistochemistry Elisa and RT-PCR 

Notes

Acknowledgments

The Translational Psychiatry Program is funded by the Department of Psychiatry and Behavioral Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth). National Institute for Molecular Medicine (INCT-MM) and Center of Excellence in Applied Neurosciences of Santa Catarina (NENASC).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Pavani Sayana
    • 1
  • Jean Pierre Oses
    • 2
  • Tatiana Barichello
    • 3
    • 4
  • Vijayasree V. Giridharan
    • 1
    Email author
  1. 1.Translational Psychiatry Program, Department of Psychiatry and Behavioral SciencesMcGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth)HoustonUSA
  2. 2.Postgraduate Program in Health and BehaviorCenter for Life Sciences and Health, Catholic University of Pelotas (UCPel)PelotasBrazil
  3. 3.Department of Psychiatry and Behavioral SciencesThe University of Texas Health Science Center at Houston (UTHealth)HoustonUSA
  4. 4.Graduate Program in Health SciencesUniversity of Southern Santa Catarina (UNESC)CriciúmaBrazil

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