Molecular Imaging of Blood–Brain Barrier Permeability in Preclinical Models Using PET and SPECT

  • Vijayasree V. Giridharan
  • Tatiana Barichello
  • Sudhakar Selvaraj
Part of the Neuromethods book series (NM, volume 142)


The blood–brain barrier (BBB) with tightest junction separates the systemic circulation and brain micro-environment to protect the brain from insults, such as infections. The integrity of BBB is preserved by multi-structural and functional components. Increasing evidence indicates that BBB is used as an important marker measured in variety of pathological condition with large permeability leaks, such as brain tumors and multiple sclerosis, to more subtle disruption such as vascular diseases, cognitive decline, and dementia. Several imaging modalities are available to study disruption of the BBB. In this chapter, we described the protocols for nuclear imaging studies such as positron emission tomography (PET) and single photon emission computed tomography (SPECT) investigating BBB permeability in preclinical models.

Key words

Blood–brain barrier Positron emission tomography Single photon emission computed tomography 



The Translational Psychiatry Program is funded by the Department of Psychiatry and Behavioral Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth). National Institute for Molecular Medicine (INCT-MM) and Center of Excellence in Applied Neurosciences of Santa Catarina (NENASC).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Vijayasree V. Giridharan
    • 4
  • Tatiana Barichello
    • 1
    • 2
  • Sudhakar Selvaraj
    • 3
  1. 1.Department of Psychiatry & Behavioral SciencesThe University of Texas Health Science Center at Houston (UTHealth)HoustonUSA
  2. 2.Graduate Program in Health SciencesUniversity of Southern Santa Catarina (UNESC)CriciúmaBrazil
  3. 3.Department of Psychiatry and Behavioral SciencesMcGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth)HoustonUSA
  4. 4.Translational Psychiatry Program, Department of Psychiatry and Behavioral SciencesMcGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth)HoustonUSA

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