Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy

  • Aline Silva de Miranda
  • Thiago Macedo Cordeiro
  • Milene Alvarenga Rachid
  • Antônio Lúcio Teixeira
Part of the Neuromethods book series (NM, volume 142)


Leukocyte infiltration in the central nervous system (CNS) has been implicated in several neuroinflammatory diseases, being an important step in the development of inflammatory response in the brain. The use of intravital microscopy technique allows direct in vivo assessment of leukocyte rolling and adhesion on cortical meningeal vessels. The current review highlights the use of intravital microscopy and its association with neuroinflammatory parameters in different models of experimental brain diseases such as HSV-1 encephalitis, dengue encephalitis, cerebral malaria, and hepatic encephalopathy. Herein, we also describe a detailed protocol of intravital microscopy of meningeal vessels, its advantages and major concerns.

Key words

Brain Pia mater vessels Intravital microscopy Leukocyte rolling Leukocyte adhesion Chemokines Mice 



The authors would like to thank FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for financial support. ASM is a 2016 NARSAD Young Investigator Grant Awardee from the Brain and Behavior Research Foundation.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Aline Silva de Miranda
    • 1
    • 2
  • Thiago Macedo Cordeiro
    • 1
  • Milene Alvarenga Rachid
    • 3
  • Antônio Lúcio Teixeira
    • 1
    • 4
  1. 1.Laboratório Interdisciplinar de Investigação Médica, Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo HorizonteBrasil
  2. 2.Laboratório de Neurobiologia, Departamento de MorfologiaInstituto de Ciências Biológicas, Universidade Federal de Minas GeraisBelo HorizonteBrasil
  3. 3.Departamento de Patologia GeralUniversidade Federal de Minas GeraisBelo HorizonteBrasil
  4. 4.Neuropsychiatry Program, Department of Psychiatry & Behavioral SciencesMcGovern Medical School, University of Texas Health Science Center at HoustonHoustonUSA

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