Evans Blue-Albumin as a Marker to Evaluate Blood-Brain Barrier Integrity in Neonatal and Adult Rodents

  • Fabricia Petronilho
  • Julia L. Goldman
  • Tatiana BarichelloEmail author
Part of the Neuromethods book series (NM, volume 142)


There has been an increase in the study of brain barriers and the several roles that they may play in neurological disorders. This type of research requires appropriate models and markers to demonstrate the integrity of the interface between the blood and the brain. Historically, dyes have been used to analyze the blood-brain barrier (BBB) mechanisms and for measurements of plasma volume. Despite some limitations, Evans blue is still the most commonly used marker for investigations of brain barrier integrity in in vivo applications. In this book chapter, we describe a simple and reproducible method for the evaluation of BBB integrity using Evans blue as a marker. Our protocol is focused on the evaluation of BBB integrity in neonatal and adult rodents, and the methods are divided into two protocols.

Key words

Blood-brain barrier Evans blue Rodent 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Fabricia Petronilho
    • 1
  • Julia L. Goldman
    • 2
  • Tatiana Barichello
    • 3
    • 4
    Email author
  1. 1.Laboratory of Neurobiology of Inflammatory and Metabolic ProcessesUniversity of Southern Santa CatarinaTubarãoBrazil
  2. 2.Center for Laboratory Animal Medicine and CareThe University of Texas Health Science Center at HoustonHoustonUSA
  3. 3.Department of Psychiatry and Behavioral SciencesThe University of Texas Health Science Center at Houston (UTHealth)HoustonUSA
  4. 4.Graduate Program in Health SciencesUniversity of Southern Santa Catarina (UNESC)CriciúmaBrazil

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