Barley pp 37-52 | Cite as

Barley Anther Culture

  • Ludmila Ohnoutkova
  • Tomas Vlcko
  • Mentewab Ayalew
Part of the Methods in Molecular Biology book series (MIMB, volume 1900)


The production of doubled haploid (DH) barley plants through anther culture is a very useful yet simple in vitro technique. DH plants derive from divisions of haploid microspores that have undergone a developmental switch under the appropriate conditions. The successive divisions lead to the formation of an embryo or callus rather than the formation of mature pollen grains. Plants that regenerate from these embryos are often either haploid, in which case their chromosome set can be doubled by treatment with colchicine, or spontaneous double haploids. The efficiency of DH plant production is highly variable depending on the genotype of the source material. Despite this limitation, DH plants have been widely used in breeding and research programs. Compared to conventional approaches, breeding strategies that makes use of DH plants achieve a homozygous state, allowing transgene or mutation stabilization in the genome, within a considerably shorter time, thus accelerating workflow or reducing work volume.

Key words

Barley (Hordeum vulgareAnther culture Doubled haploid Androgenesis 



This work was supported by the Ministry of Education, Youth and Sports, Czech Republic (Grant LO1204 from the National Program of Sustainability I.). We would like to thank the Gordon-Zeto Center for Global Education at Spelman College for supporting Tomas Vlcko and thus facilitating our collaboration. The authors are grateful to Ota Blahousek for the technical assistance.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ludmila Ohnoutkova
    • 1
  • Tomas Vlcko
    • 1
  • Mentewab Ayalew
    • 2
  1. 1.Faculty of Science, Centre of the Region Hana for Biotechnological and Agricultural Research, Department of Chemical Biology and GeneticsPalacky UniversityOlomoucCzech Republic
  2. 2.Biology DepartmentSpelman CollegeAtlantaUSA

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