Introduction of Phage Genome into Escherichia coli by Electroporation

  • Nika JanežEmail author
  • Saša Haberl Meglič
  • Karel Flisar
  • Damijan Miklavčič
  • Matjaž Peterka
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)


Electroporation has been an established tool for DNA delivery into prokaryotic and eukaryotic cells, thus facilitating basic research studies and improving medical treatments. Here we describe its use for introduction of phage genomic DNA into Escherichia coli cells, including preparation of electrocompetent cells, electric pulse optimization and recovery of electrotransformed cells. The technique can also be adapted for other bacterial species.

Key words

Phage genome Electrotransformation Bacteria 


  1. 1.
    Kotnik T, Kramar P, Pucihar G, Miklavčič D, Tarek M (2012) Cell membrane electroporation—Part 1: the phenomenon. IEEE Electr Insul Mag 28:14–23CrossRefGoogle Scholar
  2. 2.
    Satkauskas S, Ruzgys P, Venslauskas MS (2012) Towards the mechanisms for efficient gene transfer into cells and tissues by means of cell electroporation. Expert Opin Biol Ther 12:275–286CrossRefGoogle Scholar
  3. 3.
    Lu Y-P, Zhang C, Lv FX, Bie XM, Lu Z-X (2012) Study on the electro-transformation conditions of improving transformation efficiency for Bacillus subtilis. Lett Appl Microbiol 55:9–14CrossRefGoogle Scholar
  4. 4.
    Rivera AL, Magaña-Ortíz D, Gómez-Lim M, Fernández F, Loske AM (2014) Physical methods for genetic transformation of fungi and yeast. Phys Life Rev 11:184–203CrossRefGoogle Scholar
  5. 5.
    Dower WJ, Chassy BM, Trevors JT, Blaschek HP (1992) Protocols for the transformation of bacteria by electroporation. In: Chang DC, Saunders JA, Chassy BM, Sowers AE (eds) Guide to electroporation and electrofusion. Academic Press, San DiegoGoogle Scholar
  6. 6.
    Pucihar G, Krmelj J, Rebersek M, Batista Napotnik T, Miklavcic D (2011) Equivalent pulse parameters for electroporation. IEEE Trans Biomed Eng 58:3279–3288CrossRefGoogle Scholar
  7. 7.
    Lelieveld HLM, Notermans S, de Haan SWH (eds) (2007) Food preservation by pulsed electric fields. Woodhead Publishing, AbingtonGoogle Scholar
  8. 8.
    Flisar K, Haberl Meglič S, Morelj J, Golob J, Miklavčič D (2014) Testing a prototype pulse generator for a continuous flow system and its use for E. coli inactivation and microalgae lipid extraction. Bioelectrochemistry 100:44–51CrossRefGoogle Scholar
  9. 9.
    Kropinski AM, Mazzocco A, Waddell TE, Lingohr E, Johnson RP (2009) Enumeration of bacteriophages by double agar overlay plaque assay. In: Clokie MRJ, Kropinski AM (eds) Bacteriophages: volume 1: isolation, characterisation and interactions. Humana Press, New YorkGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Nika Janež
    • 1
    Email author
  • Saša Haberl Meglič
    • 2
  • Karel Flisar
    • 2
  • Damijan Miklavčič
    • 2
  • Matjaž Peterka
    • 1
  1. 1.Centre of Excellence for Biosensors, Instrumentation and Process Control, Centre for BiotechnologyAjdovscinaSlovenia
  2. 2.Laboratory of Biokibernetics, Department of Biomedical Engineering, Faculty of Electrical EngineeringUniversity of LjubljanaLjubljanaSlovenia

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