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Introduction of Phage Genome into Escherichia coli by Electroporation

  • Nika JanežEmail author
  • Saša Haberl Meglič
  • Karel Flisar
  • Damijan Miklavčič
  • Matjaž Peterka
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)

Abstract

Electroporation has been an established tool for DNA delivery into prokaryotic and eukaryotic cells, thus facilitating basic research studies and improving medical treatments. Here we describe its use for introduction of phage genomic DNA into Escherichia coli cells, including preparation of electrocompetent cells, electric pulse optimization and recovery of electrotransformed cells. The technique can also be adapted for other bacterial species.

Key words

Phage genome Electrotransformation Bacteria 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Nika Janež
    • 1
    Email author
  • Saša Haberl Meglič
    • 2
  • Karel Flisar
    • 2
  • Damijan Miklavčič
    • 2
  • Matjaž Peterka
    • 1
  1. 1.Centre of Excellence for Biosensors, Instrumentation and Process Control, Centre for BiotechnologyAjdovscinaSlovenia
  2. 2.Laboratory of Biokibernetics, Department of Biomedical Engineering, Faculty of Electrical EngineeringUniversity of LjubljanaLjubljanaSlovenia

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