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Engineering Bacteriophage-Based Biosensors

  • Daniel Brownell
  • John King
  • Brian Caliando
  • Lada Sycheva
  • Michael KoerisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)

Abstract

Bacteriophages have been used for diagnostic purposes in the past, but a lack of parallelizable engineering methods had limited their applicability to a narrow subset of diagnostic settings. More recently, however, advances in DNA sequencing and the introduction of more sensitive reporter systems have enabled novel engineering methods, which in turn have broadened the scope of modern phage diagnostics. Here we describe advanced methods to engineer the genomes of bacteriophages in a modular and rapid fashion.

Key words

Bacteriophage engineering Genome engineering Reporter systems Diagnostics Luciferase 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Daniel Brownell
    • 1
  • John King
    • 1
  • Brian Caliando
    • 1
  • Lada Sycheva
    • 1
  • Michael Koeris
    • 1
    Email author
  1. 1.Sample 6 TechnologiesWoburnUSA

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