Bacteriophages pp 199-205 | Cite as

Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy

  • Angélina Trotereau
  • Catherine SchoulerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)


To combat infectious diseases induced by antibiotic-resistant bacteria in human and animals, phage therapy has regained attention by the scientific community. Before phages can be widely accepted as therapeutics in the same way as antibiotics, convincing detailed applied experimental evidence must be available. The embryonated chicken egg model has been used to study the virulence of many pathogens. We describe here a procedure to test the efficacy of phage therapy to treat colibacillosis using a chicken embryo lethality assay, this being potentially applied to others bacterial infection.

Key words

Animal model Eggs Phage therapy Gallus gallus Escherichia coli 



We would like to thank the personnel at PFIE (plateforme d’infectiologie expérimentale) from INRA Val de Loire for providing the SPF embryonated eggs.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.ISP, INRA, Université de Tours, UMR 1282NouzillyFrance

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