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Bacteriophages pp 183-190 | Cite as

Exploitation of Drosophila Infection Models to Evaluate Antibacterial Efficacy of Phages

  • Hye-Jeong Jang
  • Hee-Won Bae
  • You-Hee ChoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)

Abstract

Nonmammalian infection models have been exploited to understand the various aspects of host-pathogen interactions and also provided innovative research platforms for identification of virulence factors, screening for antimicrobial hits, and evaluation of antimicroial efficacy. Here we describe a relatively straightforward protocol to assess the antibacterial efficacy of bacteriophages (phages) toward the opportunistic human pathogen, Pseudomonas aeruginosa, based on the systemic infection model using the fruit fly, Drosophila melanogaster. Since phages, unlike antibacterial chemicals, can be easily and sensitively enumerated by simple assays, it is also possible to address the pharmacokinetic properties of administered phages even in this small-scale infection model.

Key words

Small-scale Infection model Drosophila melanogaster Pseudomonas aeruginosa Phage Antibacterial efficacy Pharmacokinetics 

Notes

Acknowledgments

We are grateful to the members of the Cho Lab for their technical assistance and helpful comments. This work was supported by the National Research Foundation of Korea (NRF) Grant (NRF-2017R1A2B3005239).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pharmacy, College of Pharmacy and Institute of Pharmaceutical SciencesCHA UniversityGyeonggi-doSouth Korea

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