Bacteriophages pp 137-146 | Cite as

Techniques to Assess Phage–Biofilm Interaction

  • Diana Vilas Boas
  • Carina Almeida
  • Nuno Azevedo
  • Sanna Sillankorva
  • Joana AzeredoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1898)


Biofilms are ubiquitous in nature found on nearly every type of living and inert surface. They basically consist of microorganisms attached to surfaces and surrounded by a self-produced matrix of extracellular polymeric substances. Phages have proven to be successful in controlling biofilms. Here, we describe methods to characterize phage–biofilm interactions, specifically to assess biofilm biomass and to visualize the biofilm structure, discriminating infected cells using targeted molecular probes.

Key words

Bacteriophage Bacteria Biofilm Control Microscopy 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Diana Vilas Boas
    • 1
  • Carina Almeida
    • 1
    • 2
  • Nuno Azevedo
    • 3
  • Sanna Sillankorva
    • 1
  • Joana Azeredo
    • 1
    Email author
  1. 1.Laboratório de Investigação em Biofilmes Rosário Oliveira (LIBRO), Centre of Biological Engineering (CEB)University of MinhoBragaPortugal
  2. 2.Instituto Nacional de Investigação Agrária e VeterináriaVairãoPortugal
  3. 3.Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia da Universidade do PortoUniversidade do PortoPortoPortugal

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