In Vitro Suppression of CD4+ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells
Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the assessment of their ability to suppress CD4+ T-cell responses in vitro.
Key wordsMyeloid-derived suppressor cells Cell sorting In vitro proliferation assay CD4+ T-cell isolation Carboxyfluorescein succinimidyl ester (CFSE)
This work was supported by grant from the Greek General Secretariat of Research and Technology (Synergasia 09SYN-12-1074 to P.V.).