Advertisement

In Vitro Suppression of CD4+ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells

  • Katerina Vlachou
  • Panayotis VerginisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1899)

Abstract

Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the assessment of their ability to suppress CD4+ T-cell responses in vitro.

Key words

Myeloid-derived suppressor cells Cell sorting In vitro proliferation assay CD4+ T-cell isolation Carboxyfluorescein succinimidyl ester (CFSE) 

Notes

Acknowledgment

This work was supported by grant from the Greek General Secretariat of Research and Technology (Synergasia 09SYN-12-1074 to P.V.).

References

  1. 1.
    Gabrilovich DI, Nagaraj S (2009) Myeloid-derived suppressor cells as regulators of the immune system. Nat Rev Immunol 9(3):162–174PubMedPubMedCentralCrossRefGoogle Scholar
  2. 2.
    Cripps JG, Gorham JD (2011) MDSC in autoimmunity. Int Immunopharmacol 11(7):789–793PubMedPubMedCentralCrossRefGoogle Scholar
  3. 3.
    Nagaraj S, Collazo M, Corzo CA et al (2009) Regulatory myeloid suppressor cells in health and disease. Cancer Res 69(19):7503–7506PubMedPubMedCentralCrossRefGoogle Scholar
  4. 4.
    Youn JI, Nagaraj S, Collazo M et al (2008) Subsets of myeloid-derived suppressor cells in tumor-bearing mice. J Immunol 181(8):5791–5802PubMedPubMedCentralCrossRefGoogle Scholar
  5. 5.
    Poschke I, Mougiakakos D, Hansson J et al (2010) Immature immunosuppressive CD14+HLA-DR−/low cells in melanoma patients are Stat3hi and overexpress CD80, CD83, and DC-sign. Cancer Res 70(11):4335–4345PubMedCrossRefGoogle Scholar
  6. 6.
    Ioannou M, Alissafi T, Lazaridis I et al (2012) Crucial role of granulocytic myeloid-derived suppressor cells in the regulation of central nervous system autoimmune disease. J Immunol 188(3):1136–1146PubMedCrossRefGoogle Scholar
  7. 7.
    Kusmartsev S, Nefedova Y, Yoder D et al (2004) Antigen-specific inhibition of CD8+ T cell response by immature myeloid cells in cancer is mediated by reactive oxygen species. J Immunol 172(2):989–999PubMedCrossRefGoogle Scholar
  8. 8.
    Nagaraj S, Youn JI, Gabrilovich DI (2013) Reciprocal relationship between myeloid-derived suppressor cells and T cells. J Immunol 191(1):17–23PubMedPubMedCentralCrossRefGoogle Scholar
  9. 9.
    Ioannou M, Alissafi T, Boon L et al (2013) In vivo ablation of plasmacytoid dendritic cells inhibits autoimmunity through expansion of myeloid-derived suppressor cells. J Immunol 190(6):2631–2640PubMedPubMedCentralCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Autoimmunity and InflammationInstitute of Molecular Biology and Biotechnology, Foundation for Research and TechnologyHeraklionGreece
  2. 2.Laboratory of Cellular Immunology and Immune Tolerance, Division of Immunology and TransplantationBiomedical Research Foundation Academy of AthensAthensGreece

Personalised recommendations