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Isolation and Characterisation of Human Adipose-Derived Stem Cells

  • Anna Wilson
  • Melisse Chee
  • Peter Butler
  • Ashleigh S. BoydEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1899)

Abstract

Recently, adipose-derived stem cells (ASCs), obtained from fresh human lipoaspirate, have shown promise as immunomodulatory agents having demonstrated immunosuppressive functionality both in vitro and in vivo. A number of researchers have described the isolation of ASCs through the enzymatic digestion of fat samples, followed by a number of purification steps, involving centrifugation and filtration. Here, we utilize a standard isolation technique, with the added purification of putative ASCs by fluorescence activated cell sorting (FACS). ASCs are an extremely valuable resource in clinical applications, including reconstruction, regeneration, and investigations into immune activity. This method could be used to isolate and purify ASCs for such downstream applications.

Key words

Adipose-derived stem cells Adipose stem cells ASCs Isolation Characterization Immunosuppression Immunomodulation Stromal vascular fraction Collagenase Lipotransfer Lipoaspirate 

Notes

Acknowledgments

This work was supported by the Restoration of Appearance and Function Trust (RAFT), with funding from the Kirby Laing Foundation.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Anna Wilson
    • 1
  • Melisse Chee
    • 1
  • Peter Butler
    • 1
  • Ashleigh S. Boyd
    • 2
    • 3
    Email author
  1. 1.Research Department of Surgical Biotechnology, UCL Division of Surgery and Interventional Science, University College LondonRoyal Free HospitalLondonUK
  2. 2.Associate Professor in Stem Cell Biology and Regenerative Medicine, Research Department of Surgical Biotechnology Division of Surgery and Interventional Science University College London (UCL)Royal Free HospitalLondonUK
  3. 3.UCL Institute of Immunity and TransplantationRoyal Free HospitalLondonUK

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