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Alarmin Detection in Senescent Cells

  • Dong Eun Kim
  • Albert R. Davalos
Part of the Methods in Molecular Biology book series (MIMB, volume 1896)

Abstract

Senescent cells secrete diverse array of proteins. One group of proteins, damage-associated molecular pattern (DAMP) proteins exhibit relocalization from inside to outside the cell. High Mobility Group Box 1 protein (HMGB1) is the founding DAMP member. HMGB1 relocalization from the nucleus provides a molecular signature during senescence. We provide distinct molecular techniques (immunofluorescence, immunohistochemistry, and Western blot assays) to assess HMGB1 relocalization during the initial stages of senescence.

Key words

Damage-associated molecular pattern (DAMP) Alarmin High Mobility Group Box 1 protein (HMGB1) Immunofluorescence Immunohistochemistry Protein precipitation Western blot 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Buck Institute for Research on AgingNovatoUSA

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