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Measuring the Inflammasome in Oncogene-Induced Senescence

  • Irene Fernández-Duran
  • Núria Tarrats
  • Priya Hari
  • Juan Carlos Acosta
Part of the Methods in Molecular Biology book series (MIMB, volume 1896)

Abstract

Inflammasomes are multimeric protein complexes that process IL-1β by cleaving the translated full-length protein into its active IL-1β mature fragment. In oncogene-induced senescence, inflammasomes play a crucial role by regulating IL1R signaling and consequently modulating proliferation and the senescence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of IL1B expression, followed by (b) cleavage and release of mature IL-1β. In this chapter, we describe methods to detect both stages of inflammasome activation in cellular senescence.

Key words

Senescence Inflammasome Caspase-1 IL-1β SASP Oncogene-induced senescence 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Irene Fernández-Duran
    • 1
  • Núria Tarrats
    • 1
  • Priya Hari
    • 1
  • Juan Carlos Acosta
    • 1
  1. 1.Edinburgh Cancer Research UK Centre, Institute of Genetics and Molecular MedicineUniversity of EdinburghEdinburghUK

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