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Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Vivo

  • Bennett G. Childs
  • Tyler J. Bussian
  • Darren J. Baker
Part of the Methods in Molecular Biology book series (MIMB, volume 1896)

Abstract

Senescence-associated β-galactosidase (hereafter SA-β-gal) staining has now been employed for more than 20 years to identify the presence of senescent cells (Dimri et al., Proc Natl Acad Sci U S A 92:9363–9367, 1995). These cells, characterized by a permanent cell-cycle arrest (Hayflick and Moorhead, Exp Cell Res 25:585–621, 1961) and the production of a distinct secretory phenotype of cytokines, chemokines, and proteases (Coppe et al., PLoS Biol 6:2853–2868, 2008), have received much attention in recent years for their impacts on diverse biological processes. Here we describe a method to identify and quantify the specific cells that become senescent in vivo using transmission electron microscopy after SA-β-gal staining that can be used in countless scenarios.

Key words

Cellular senescence Transmission electron microscopy Senescence-associated β-galactosidase 

Notes

Acknowledgments

This work was supported by the National Institutes of Health (R01AG053229), the Glenn Foundation for Medical Research, the Ellison Medical Foundation, and the Mayo Clinic Children’s Research Center to D.J.B.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Bennett G. Childs
    • 1
  • Tyler J. Bussian
    • 1
  • Darren J. Baker
    • 1
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyMayo ClinicRochesterUSA
  2. 2.Department of Pediatric and Adolescent MedicineMayo ClinicRochesterUSA

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