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A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in Fluids

  • Sophia V. Rizou
  • Konstantinos Evangelou
  • Vassilios Myrianthopoulos
  • Iordanis Mourouzis
  • Sophia Havaki
  • Aikaterini Athanasiou
  • Panagiotis V. S. Vasileiou
  • Aggelos Margetis
  • Athanassios Kotsinas
  • Nikolaos G. Kastrinakis
  • Petros Sfikakis
  • Paul Townsend
  • Emmanuel Mikros
  • Constantinos Pantos
  • Vassilis G. Gorgoulis
Part of the Methods in Molecular Biology book series (MIMB, volume 1896)

Abstract

Lipofuscin accumulation is a hallmark of senescence. This nondegradable material aggregates in the cytoplasm of stressed or damaged cells due to metabolic imbalance associated with aging and age-related diseases. Indications of a soluble state of lipofuscin have also been provided, rendering the perspective of monitoring such processes via lipofuscin quantification in liquids intriguing. Therefore, the development of an accurate and reliable method is of paramount importance. Currently available assays are characterized by inherent pitfalls which demote their credibility. We herein describe a simple, highly specific and sensitive protocol for measuring lipofuscin levels in any type of liquid. The current method represents an evolution of a previously described assay, developed for in vitro and in vivo senescent cell recognition that exploits a newly synthesized Sudan Black-B analog (GL13). Analysis of human clinical samples with the modified protocol provided strong evidence of its usefulness for the exposure and surveillance of age-related conditions.

Key words

Lipofuscin GL13 (SenTraGorSenescence Biological fluids Aging Age-related diseases 

Notes

Acknowledgments

We would like to thank Dr. Alexandros Papalampros and Dr. Dimitrios Papadopoulos for providing material for this investigation. This work was financially supported by the “SYNTRAIN” ITN Horizon 2020 Grant No 722729, the NKUA SARG grants 70/3/12128, 70/3/8916, 70/3/1135 and the Welfare Foundation for Social & Cultural Sciences (KIKPE) Greece.

Conflict of interest:

The authors wish to declare no conflict of interest.

Patent pending: UK Patent Application No GB1803531.1.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sophia V. Rizou
    • 1
  • Konstantinos Evangelou
    • 1
    • 2
  • Vassilios Myrianthopoulos
    • 3
    • 4
  • Iordanis Mourouzis
    • 5
  • Sophia Havaki
    • 1
  • Aikaterini Athanasiou
    • 6
  • Panagiotis V. S. Vasileiou
    • 1
  • Aggelos Margetis
    • 1
  • Athanassios Kotsinas
    • 1
  • Nikolaos G. Kastrinakis
    • 1
  • Petros Sfikakis
    • 7
  • Paul Townsend
    • 8
  • Emmanuel Mikros
    • 3
    • 4
  • Constantinos Pantos
    • 5
  • Vassilis G. Gorgoulis
    • 1
    • 8
    • 9
    • 10
  1. 1.Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical SchoolNational and Kapodistrian University of AthensAthensGreece
  2. 2.Department of Anatomy-Histology-Embryology, Medical SchoolUniversity of IoanninaIoanninaGreece
  3. 3.Division of Pharmaceutical Chemistry, School of PharmacyNational and Kapodistrian University of AthensAthensGreece
  4. 4.PharmaInformatics UnitAthena Research CenterAthensGreece
  5. 5.Department of Pharmacology, Medical SchoolNational and Kapodistrian University of AthensAthensGreece
  6. 6.Department of Obstetrics and GynecologyWeill Cornell MedicineNew YorkUSA
  7. 7.First Department of Propaedeutic Internal Medicine and Rheumatology Unit, Medical SchoolNational and Kapodistrian University of AthensAthensGreece
  8. 8.Faculty Institute for Cancer Sciences, Manchester Academic Health Sciences CentreUniversity of ManchesterManchesterUK
  9. 9.Biomedical Research FoundationAcademy of AthensAthensGreece
  10. 10.Center for New Biotechnologies and Precision MedicineMedical School, National and Kapodistrian University of AthensAthensGreece

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