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Methods for Evaluation of Ovarian Granulosa Cells with Exposure to Nanoparticles

  • Jin Liu
  • Wenchang Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1894)

Abstract

Increasing evidence demonstrates that a substantial number of chemicals including nanoparticles have potential toxic effects on human and wildlife reproductive health. Ovarian granulosa cells (GCs), the functional unit of the ovary, play a pivotal role in the regulation of ovarian reproductive health in females. They are considered to be the main target by the chemicals at all stages of their development. It has been reported that nanoparticles induced toxicity of GCs by interfering with cell cycle. Some basic methods for evaluation of GCs with exposure to nanoparticles are described in this chapter including isolation of GCs, determination of apoptosis by immunofluorescence, and measurement of progesterone level by ELISA.

Key words

Ovarian granulosa cells In vitro culture Immunofluorescence ELISA Nanoparticles 

Notes

Acknowledgments

This work was supported by NSFC grant (no. 81673212, 81373027, 30972514) and Fujian Provincial Key Laboratory of Environmental Factors and Cancer.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jin Liu
    • 1
    • 2
  • Wenchang Zhang
    • 1
    • 2
  1. 1.Fujian Provincial Key Laboratory of Environmental Factors and Cancer, School of Public HealthFujian Medical UniversityFuzhouPeople’s Republic of China
  2. 2.Department of Preventive Medicine, School of Public HealthFujian Medical UniversityFuzhouPeople’s Republic of China

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