Nanotoxicity pp 345-352 | Cite as

Application of Daphnia magna for Nanoecotoxicity Study

  • Zhizhen Xu
  • Yingying Liu
  • Yuqian Wang
Part of the Methods in Molecular Biology book series (MIMB, volume 1894)


Daphnia magna (D. magna), an aquatic invertebrate, is the most commonly used test organism in ecotoxicological studies on manufactured nanomaterials (MNs). Although standard protocols for undertaking acute and chronic toxicity tests of dissolved chemicals with D. magna have been endorsed by several national and international organizations, comprehensive guidance on aquatic toxicology testing of MNs with D. magna are in their infancy. Here we describe the acute and chronic toxicity methods with D. magna as test organism for nanoecotoxicity study. These methods may provide reference for the next step toward developing prescriptive aquatic toxicity standard tests for MNs with D. magna.

Key words

Nanomaterials Daphnia magna Nanoecotoxicity Acute toxicity Chronic toxicity 



This work was supported by the Youth Core Plan of Beijing Academy of Science and Technology (No. 201703).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Zhizhen Xu
    • 1
  • Yingying Liu
    • 2
  • Yuqian Wang
    • 1
  1. 1.Key Laboratory of Occupational Safety and HealthBeijing Municipal Institute of Labour ProtectionBeijingPeople’s Republic of China
  2. 2.Department of Raw Material Industry ResearchChina Center for Information Industry DevelopmentBeijingPeople’s Republic of China

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