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Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions

  • Yurika Matsui
  • Zhi-Chun LaiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1893)

Abstract

Protein-protein interactions provide a common mechanism for regulating protein functions and also serve as the fundamental step of many biochemical reactions. To accurately determine the involvement and function of protein-protein interactions, it is crucial to detect the interactions with the minimum number of artifacts. In this chapter, we report the method of bimolecular fluorescence complementation (BiFC) in tissue culture and developing tissues of Drosophila, which allows the visualization of subcellular localization of protein-protein interactions in living cells.

Key words

Bimolecular fluorescence complementation (BiFC) Fluorescence microscopy Protein-protein interaction Tissue culture Drosophila wing imaginal discs 

Notes

Acknowledgments

We would like to thank Dr. Yaoting Deng for sharing her data on Hpo dimerization. This work was partly supported by the National Science Foundation.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Intercollege Graduate Degree Program in Molecular, Cellular and Integrative BiosciencesThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of BiologyThe Pennsylvania State UniversityUniversity ParkUSA
  3. 3.Department of Biochemistry and Molecular BiologyThe Pennsylvania State UniversityUniversity ParkUSA

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