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Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag

  • Rui Chen
  • Steven W. Plouffe
  • Kun-Liang GuanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1893)

Abstract

Protein phosphorylation is one of the most important posttranslational modifications in cell signaling and regulation. Protein phosphorylation can be detected by site-specific phospho-antibodies, but generating these antibodies can be costly, time-consuming, and difficult. Recently, Phos-tag technology has been developed to detect protein phosphorylation. Here, we describe our method for using Phos-tag gels to compare the phosphorylation status of YAP and TAZ, the most important downstream effectors of the Hippo pathway.

Key words

Hippo pathway Phos-tag gel Phosphorylation YAP TAZ 

Notes

Acknowledgments

This work was supported by grants from the National Institutes of Health (CA196878, CA217642, and GM51586) to K.-L.G. S.W.P. is supported by the UCSD Graduate Training Program in Cellular and Molecular Pharmacology (T32 GM007752). K.-L.G. is a co-founder and has an equity interest in Vivace Therapeutics, Inc. The terms of this arrangement have been reviewed and approved by the University of California, San Diego, in accordance with its conflict of interest policies.

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pharmacology and Moores Cancer CenterUniversity of CaliforniaLa JollaUSA

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