Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors
The translocation or shuttling of Hippo proteins between the nucleus and cytoplasm is a rapid event following cytoskeletal or mechanical cues as well as stimulation with extracellular growth factors. Here we describe an experimental procedure for a simple and fast separation of nuclear and cytoplasmic fractions which maintains protein integrity and integrity of protein-protein complexes, indicating that it should be applicable to many experimental questions.
Key wordsSubcellular fractionation Hippo pathway Hypotonic buffer Cytoplasmic compartment Nuclear compartment
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