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Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors

  • Maria Chatzifrangkeskou
  • Eric O’NeillEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1893)

Abstract

The translocation or shuttling of Hippo proteins between the nucleus and cytoplasm is a rapid event following cytoskeletal or mechanical cues as well as stimulation with extracellular growth factors. Here we describe an experimental procedure for a simple and fast separation of nuclear and cytoplasmic fractions which maintains protein integrity and integrity of protein-protein complexes, indicating that it should be applicable to many experimental questions.

Key words

Subcellular fractionation Hippo pathway Hypotonic buffer Cytoplasmic compartment Nuclear compartment 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of OncologyUniversity of OxfordOxfordUK

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