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Adhesion of Lactobacillus to Intestinal Mucin

  • Keita Nishiyama
  • Takao Mukai
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1887)

Abstract

Adhesion to intestinal mucin is one of the main in vitro tests for the study of probiotic strains. Mucins immobilized on microtiter plates and glass slides are easy and excellent methods used for the quantitative analysis of Lactobacillus adhesion. However, to maintain the performance of the quantitative analysis, these methods must be chosen appropriately according to the nature and characteristics of the strain, such as tolerance to surfactant and the ability to self-aggregate. Here we describe two methodologies used to evaluate adhesion of Lactobacillus to mucin, in addition to the isolation and purification of mucins from porcine colonic tissues.

Key words

Adhesion Aggregation Diagnostic glass slide Mucin Microtiter plate 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Microbiology, School of PharmacyKitasato UniversityTokyoJapan
  2. 2.Department of Animal Science, School of Veterinary MedicineKitasato UniversityTowadaJapan

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