Isolation of Lactic Acid Bacteria Eliminating Trimethylamine (TMA) for Application to Fishery Processing
Fishy odor of fish flesh (meat) presents a severe problem for marine production. The main cause of fishy odor is trimethylamine (TMA), which increases during storage. It is produced from trimethylamine oxide (TMAO), an osmosis-regulating substance in fish cells that functions by a reduction reaction. Bacterial growth in fish meat increases TMA. Its odor reduces the commercial value of the meat. Technologies for its regulation and elimination are desired. This chapter presents a description of the use of lactic acid to eliminate TMA. The lactic acid is producible safely by bacteria during food processing using picric acid—toluene.
A method of eliminating TMA was demonstrated using Lactobacillus plantarum H78. Furthermore, an assay method was explained for reducing TMA in fish meat by fermenting the H78 strain.
Key wordsFish meat Lactobacillus plantarum Picric acid–toluene method Spectrophotometry Trimethylamine
- 1.Ministry of Agriculture, Forestry and Fisheries. http://www.jfa.maff.go.jp/j/kikaku/wpaper/h22_h/trend/1/t1_2_1_1.html. Accessed 20 Jan 2018
- 2.Koizumi C, Ohashi T (2005) Processing and storage of marine food products. Kouseisha Kouseikaku Co., Ltd, TokyoGoogle Scholar
- 5.Charalambous G (2013) Off-flavors in foods and beverages. Elsevier Science, Amsterdam, p 154Google Scholar
- 6.Yamamoto K, Kato T (2007) Development of unsalted fish sauce. Using antibacterial lactic acid bacteria. Annu Rep Food Res Inst 6:114–117 (in Japanese)Google Scholar
- 8.Fujii T (1975) Removal of fishy odors by microorganisms. Koryo 113:46–48Google Scholar