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Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes

  • Vanessa Henriques
  • Susana Machado
  • Wolfgang Link
  • Bibiana I. Ferreira
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1890)

Abstract

FOXO proteins represent a subfamily of transcription factors that belong to the forkhead family. The study of FOXO target genes can be performed using Real-Time PCR (RT-PCR). The RT-PCR is a sensitive method that allows the detection and quantification of minute amounts of nucleic acids. In RT-PCR the accumulation of the amplicon is detected and measured as the reaction progresses. Here, we describe the application of RT-PCR technique to monitor the transcriptional activity of FOXO transcription factors.

Key words

FOXO Transcription factor Target genes Real-time PCR Fluorescent reporter Quantitative 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Vanessa Henriques
    • 1
  • Susana Machado
    • 1
  • Wolfgang Link
    • 4
  • Bibiana I. Ferreira
    • 1
    • 2
    • 3
  1. 1.Centre for Biomedical Research (CBMR)University of AlgarveFaroPortugal
  2. 2.Regenerative Medicine Program, Department of Biomedical Sciences and MedicineUniversity of AlgarveFaroPortugal
  3. 3.Algarve Biomedical Center (ABC)University of AlgarveFaroPortugal
  4. 4.Instituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM)MadridSpain

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