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Hydra as Model to Determine the Role of FOXO in Longevity

  • Thomas C. G. Bosch
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1890)

Abstract

In non-senescent Hydra, continuously high activity of transcription factor FOXO contributes to continuous stem cell proliferation. Here, we describe how genetic manipulation of Hydra polyps using embryo-microinjection allows uncovering the role of FOXO in coordinating both stem cell proliferation and antimicrobial peptide0073 , effector molecules of the innate immune system, and regulators of the microbiome.

Key words

Hydra Stem cells Aging Microbiome FOXO Embryo microinjection Reporter gene constructs Antimicrobial peptides (AMPs) 
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Notes

Acknowledgment

This work was supported by the Deutsche Forschungsgemeinschaft (DFG) (CRC1182 “Origin and Function of Metaorganisms,” DFG grant BO 848/17-1, and grants from the DFG Cluster of Excellence program “Inflammation at Interfaces”). The author gratefully appreciates support from the Canadian Institute for Advanced Research (CIFAR).

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Zoological InstituteKiel UniversityKielGermany

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