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RNAi-Mediated Silencing of FOXO Factors

  • Priyankar Sanphui
  • Akash Saha
  • Subhas Chandra BiswasEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1890)

Abstract

RNAi-mediated silencing of a particular gene is an important tool in the field of biology. Knocking down experiments has revealed the role of different proteins and their downstream targets. Here, we describe the procedure of silencing transcription factor of Forkhead box class “O” (FOXO) using shRNA.

Key words

FOXO shRNA Competent cell Transformation Transfection 

Notes

Acknowledgement

The work was supported by one of the 12th Five Year Plan Projects, miND (BSC0115) of CSIR, Govt. of India.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Priyankar Sanphui
    • 1
    • 2
  • Akash Saha
    • 1
  • Subhas Chandra Biswas
    • 1
    Email author
  1. 1.Cell Biology and Physiology DivisionCSIR-Indian Institute of Chemical BiologyKolkataIndia
  2. 2.Department of ZoologySree Chaitanya CollegeHabraIndia

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