Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors

  • Yong YuEmail author
  • Pengtao LiuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1884)


Single-cell RNA sequencing (scRNA-seq) is a powerful tool to study immune cells, which enables an unbiased way to discover novel cell populations, biological meaningful cellular heterogeneity, and cell lineage development trajectories. Advances in scRNA-seq technologies and computational data analysis have driven a revolution in our understanding of the immune system in health and disease. Technically, the key step for scRNA-seq analysis is making a high-quality cDNA library for sequencing. Here, we describe a plate-based protocol to prepare single-cell cDNA library of bone marrow innate lymphoid precursors for next generation sequencing-based transcriptome analysis.

Key words

scRNA-seq Innate lymphoid cells Cellular heterogeneity Immune cell development PD-1 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, School of Life Sciences and Technology, Tongji UniversityShanghaiChina
  2. 2.LKS Faculty of MedicineSchool of Biomedical Sciences, The University of Hong KongHong KongChina

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