Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors
Single-cell RNA sequencing (scRNA-seq) is a powerful tool to study immune cells, which enables an unbiased way to discover novel cell populations, biological meaningful cellular heterogeneity, and cell lineage development trajectories. Advances in scRNA-seq technologies and computational data analysis have driven a revolution in our understanding of the immune system in health and disease. Technically, the key step for scRNA-seq analysis is making a high-quality cDNA library for sequencing. Here, we describe a plate-based protocol to prepare single-cell cDNA library of bone marrow innate lymphoid precursors for next generation sequencing-based transcriptome analysis.
Key wordsscRNA-seq Innate lymphoid cells Cellular heterogeneity Immune cell development PD-1
- 6.Yu Y, Tsang JC, Wang C, Clare S, Wang J, Chen X, Brandt C, Kane L, Campos LS, Lu L, Belz GT, McKenzie AN, Teichmann SA, Dougan G, Liu P (2016) Single-cell RNA-seq identifies a PD-1(hi) ILC progenitor and defines its development pathway. Nature 539(7627):102–106. https://doi.org/10.1038/nature20105CrossRefPubMedGoogle Scholar